Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase
نویسندگان
چکیده
منابع مشابه
Liver cytosol tyrosine aminotransferase. Product inhibition and interaction with substrates.
The reaction catalyzed by purified rat liver cytosol tyrosine aminotransferase (L-tyrosine + 2 oxoglutarate = p-hydroxyphenylpyruvate + L-glutamate, EC 2.6.1.5) has been analyzed with regard to product inhibition and combination with each of its rate-limiting substrates by the Hill equation. Glutamate is a linear competitive inhibitor with respect to tyrosine, a linear noncompetitive inhibitor ...
متن کاملA recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities.
Tyrosine aminotransferase purified from epimastigotes of Trypanosoma cruzi displays an additional activity of alanine aminotransferase, absent in all other tyrosine aminotransferases characterized so far. Since the parasite's genome contains a high number of copies of the tyrosine aminotransferase gene, we could not rule out the possibility that two very similar proteins, with changed specifici...
متن کاملRationally designed complex, hierarchical microarchitectures.
The emergence of complex nano- and microstructures is of fundamental interest, and the ability to program their form has practical ramifications in fields such as optics, catalysis, and electronics. We developed carbonate-silica microstructures in a dynamic reaction-diffusion system that allow us to rationally devise schemes for precisely sculpting a great variety of elementary shapes by diffus...
متن کاملRationally designed tumor-penetrating nanocomplexes.
Small interfering RNA (siRNA) therapeutics have broad potential uses in medicine but require safe and effective delivery vehicles to function. An ideal delivery system should encapsulate and protect the siRNA cargo from serum proteins, exhibit target tissue and cell specificity, penetrate the cell surface, and release its cargo in the desired intracellular compartment. One approach to the desig...
متن کاملIn vivo continuous directed evolution.
The development and application of methods for the laboratory evolution of biomolecules has rapidly progressed over the last few decades. Advancements in continuous microbe culturing and selection design have facilitated the development of new technologies that enable the continuous directed evolution of proteins and nucleic acids. These technologies have the potential to support the extremely ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Science
سال: 2004
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.03117204